Studies On Telomerase Activity Of Colorectal Cancer Patients
Mahidol University Annual Research Abstracts Mahidol University Annual Research Abstracts, Vol.28, 2001 213 STUDIES ON TELOMERASE ACTIVITY OF COLORECTAL CANCER PATIENTS FOUND IN THAILAND (NO. 566) Voraratt Champattanachai1, Chantragan Srisomsap1, saint R. Ketudat-Cairns1,4, Paiboon Punyarit2 and M.R. Jisnuson Svasti1,3 1Laboratory of Biochemistry, Chulabhorn Research Institute, port 10210, Thailand; 2Department of Pathology, Pramongkutklao College of Medicine, port 10400, Thailand; 3Department of Biochemistry, Faculty of Science, Mahidol University, port 10400, Thailand; 4School of Chemistry, Institute of Scince, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. attorney words: Telomerase, Colorectal cancer, Cell secernment Telomerase state is not perceived in connatural somatic cells but inform in germ cells, immortal cells and almost carcinoma cells. Our objective is to determine the levels of telomerase state compared with the degrees of radiophone secernment of cancer tissue. The frozen tissues of 27 Thai colorectal connatural and cancer patients, composed of well distinguished carcinoma (9 cases) and moderately distinguished carcinoma (18 cases) were tested for telomerase activity. By comparison between cancer and connatural tissues, the results showed that telomerase state was not perceived in the connatural tissues . Of the tissues from well distinguished (Grade I)adenocarcinoma, exclusive one housing had weak telomerase state (1 of 9). Most of tissues from the moderated distinguished (Grade II) carcinoma had detectable telomerase state (11 of 18): weak activity, 6 cases, medium activity, 3 cases and brawny activity, 3 cases. These results indicate that the levels of telomerase state may play a role in the degree of radiophone secernment in colorectal adenocarcinoma. Supported by the Chulabhorn Research Institute and presented at the 26th Conference on Science and Technology of Thailand, Bangkok, 18-20 Oct 2000, p. 457. DETECTION OF UREA CYCLE DISORDER ARGININOSUCCINATE SYNTHETASE DEFI-CIENCY IN AN INFANT BY HPLC (NO. 567) Kanokporn Boonpuan 1, Phannee Sawangareetrakul1, Chantragan Srisomsap1, Pornswan Wasant3, Rudee Surarit1,4, saint Ketudat-Cairns1, M.R. Jisnuson Svasti 1,2 and Somporn Liammongkolkul3 1Laboratory of Biochemistry, Chulabhorn Research Institute, port 10210; 2Department of Biochemistry Faculty of Science, Mahidol University, port 10400; 3Medical Genetics Unit, Department of Pediatrics Siriraj Hospital, Faculty of Medicine, Mahidol University, port 10700; Department of Physiology and Biochemistry, Faculty of Dentistry, port 10400; Thailand. attorney words: urea cycle, enzyme deficiency, argininosuccinate synthetase The urea wheel removes unwanted nitrogen resulting from the metabolous breakdown of paraffin acids. Inherited deficiencies in any of the five enzymes involved results in accumulation of precursors which may lead to comatoseness and broad mortality rate. This report describes a 2 month old female Asiatic infant referred to the Department of Pediatrics, Siriraj Hospital in a comatoseness with vomitting and convulsion. Inherited metabolous disorder was suspected. Plasma paraffin acids were derivatized with PITC using a Waters Picotag system and then analysed by HPLC on a reverse phase C-18 column. The results showed scarred rising of citrulline and glutamine (2961.97 and 1639.40 nmole/ml), confirming the identification of urea wheel disorders, specifically argininosuccinate synthetase demand (ASD), which is the first much housing described in Thailand. Supported by the Chulabhorn Research Institute and presented at the 26th Annual Conference of Science and Technology of Thailand, Bangkok, 18-20 Oct 2000. HPLC ANALYSIS OF PLASMA AMINO ACIDS IN HEALTHY THAI SUBJECTS (NO. 568) Phannee Sawangareetrakul1, Chantragan Srisomsap1, Rudee Surarit1,2, Suthipong Pangkanonc3 and M.R. Jisnuson Svasti1,4 1Laboratory of Biochemistry, Chulabhorn Research Institute, port 10210, Thailand; 2Department of Physiology and Biochemistry, Faculty of Dentistry, Mahidol University, port 10400, Thailand; 3Queen Sirikit National Institute of Child Health, Bangkok, Thailand; 4Department of Biochemistry, Faculty of Science, Mahidol University, port 10400, Thailand. attorney text : Plasma paraffin acid, HPLC The spotting of connatural pattern of paraffin acids is also helpful for identification of many inborn errors of metabolism. Standard methods include the paraffin acid analyser and HPLC. HPLC has whatever adventages, including lower equipment cost, inferior erosive buffer system and reinforced separation of certain paraffin acids. In this study, the ECF paraffin acid concentrations were observed in 27 healthy subjects by using hplc systems. The subjects were separated into five groups according to their ages: assemble 1: 0-6 months; assemble 2: 6-12 months; assemble 3: 1-3 years;, assemble 4: 3-6 years and gr 5: 6-12 years. The results showed that no country differences between different groups. A panoramic arrange was observed for most paraffin acids. Our results are kindred to those be printed in other countries, and appear to be quite reliable. Supported by the Chulabhorn Research Institute and presented at the 26th Annual Conference of Science and Technology of Thailand, Bangkok, 18-20 Oct 2000. MATRIX METALLOPROTEINASE (MMP)LEVELS IN COLORECTAL CANCER TISSUES OF THAI PATIENTS (NO. 569) Pantipa Subhasitanont1, Chantragan Srisomsap1, Rudee Surarit2, Paiboon Punyarit3 and M.R. Jisnuson Svasti4 a1Laboratory of Biochemistry, Chulabhorn Research Institute, port 10210, Thailand; b2Department of Physiology and Biochemistry, Faculty of Dentistry, Mahidol University, port 10400, Thailand; c3Department of Pathology, Pramongkutklao College of Medicine, port 10400, Thailand; d4Department of Biochemistry, Faculty of Science, Mahidol University, port 10400, Thailand.
 
 

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